The goal of customized development of Jicang biological recombinant protein is to determine the optimal clone together with customer service. Taking E. coli recombinant expression system as an example, according to customer requirements, we started with codon optimization, vector construction and expression strain screening, and finally obtained high expression stable recombinant strains through small amount of induction detection, protein purification and identification, providing high-quality customized development services for recombinant proteins throughout the process.
01.The team has rich experience in recombinant protein system expression. It can provide one-stop technical services to deliver high-purity target proteins to customers in a short time.
E. coli expression system development service:
Rapid and low cost construction of recombinant E. coli expression system
Study on the biosafety, heredity and expression stability of recombinant strains
Establishment of three-level Library
Product quality analysis
02. the bacterial culture process development service aims to provide the best bacterial culture process for customer service. Starting from participating in the screening of optimal strains, we optimized the culture medium, inoculation density, feeding and process control strategy to maximize expression, increase process reproducibility and facilitate process amplification.
Bacterial culture process development service:
Culture of bacteria in animal free medium
Optimization and evaluation of culture medium
Process development of batch feeding
Process amplification
Preclinical bacterial culture batch production
03.Protein purification process development is committed to improving product quality and purity, and providing a stable and repeatable protein purification process. The technology and raw materials used in this process are suitable for cost-effective mass production. Now it has a fully automatic protein purification system with intelligent optimization of experimental conditions.
Purification process R & D services:
Standardized protein purification unit operation
Clarification filtration development (deep filtration, tangential flow filtration)
Optimization of chromatographic purification (support affinity chromatography, gel block chromatography, ion exchange chromatography, hydrophobic chromatography, etc.)
Process amplification